BİOMATBIOMATERIALS AND TISSUE ENGINEERING RESEARCH LABORATORY


Assist. Prof. Dr. Deniz Yücel



Title: Assist. Prof. Dr. 
Education:  Ph.D. Biotechnology, METU, 2009
M.Sc. Biotechnology, METU, 2003 
B.Sc. Biological Sciences, METU, 2001
Contact: Telephone:  
e-mail: dyucel@metu.edu.tr



Research Field:

 

Stem Cell Based Nerve Tissue Engineering on Patterned Constructs

 

Nerve tissue engineering (NTE) is one of the most promising methods to restore CNS in human health care. Three-dimensional (3-D) distribution and growth of cells within the scaffold are of clinical significance for NTE. Polymers can be used as scaffold to promote cell adhesion, maintenance of differentiated cell function without hindering proliferation, template to organize and direct the growth of cells and help in the function of extracellular matrix (ECM).

 

The scope of this study is to construct a 3-D, biodegradable, polymeric nerve tube with micropatterned films and microfibers to regenerate and restore the function of nerve tissue. The micro-fibers are produced by electrospinning technique, while the micropatterned films are obtained by solvent casting. Stem cells and supporting cells are used to obtain tissue engineered nerve construct.

 

M.Sc. Study:

 

Construction of a Choline Oxidase Biosensor

 

The scope of the study was the construction of an amperometric choline oxidase biosensor to determine choline, which is indispensable for a number of fundamental processes in the body. Choline oxidase was covalently immobilized onto HEMA-grafted membranes via a spacer. HEMA grafting was achieved by gamma irradiation. Immobilized choline oxidase studies were carried out in an oxygen electrode unit and were based on oxygen depletion in the reaction medium. In this study, the effect of activation procedure, the location of the catalytic membrane and the immobilization enzyme concentration on immobilized enzyme activity were carried out in the oxygen electrode. The performance of the choline oxidase biosensor was studied in terms of reusability, linear working range and sensitivity.

 

Academic Experience:

 

  • 2005-2006   Scientific and Technical Research Council of Turkey International PhD
  • June-Sep 2007 Grantee and Visiting Researcher at Mass General Hospital/Shriners Hospital for Children at Harvard Medical School
  • 2001- 2008 Research Assistant in Bioanalytical Chemistry and Biochemistry Laboratories, Middle East Technical University, Department of Biological Sciences, Ankara

 

Selected Publications:

 

  • Kenar H, Yucel D, Kose GT, Hasirci V.Mesenchymal Stem Cell Behaviour on Topographical Cues. TERMIS-EU 2008 Annual Meeting, 22-26 June, 2008, Porto, PORTUGAL
  • Kenar H, Yucel D, Akhmetova L, Hasirci V.Is Wharton's Jelly MSC Division Influenced by Substrate Microgrooves? 14th International Biomedical Science & Technology Symposium, 03-07 May, 2008, Muğla, TURKEY
  • Hasirci, V., Yucel, D. Polymers Used in Tissue Engineering, in Encyclopedia of Biomaterials and Biomedical Engineering 2007, 1:1, 1-17, http://www.informaworld.com/smpp/title~content=t713172959
  • Yucel, D., Ozer, N., Hasirci, V. Construction of a choline biosensor through enzyme immobilization on a poly(2-hydroxyethyl methacrylate) grafted Teflon film, J. Applied Polymer Science 2007, 1004, 3469-3477
  • Yilgör, P, Yucel, D, Kenar, H, Hasirci,V, Polyhydroxyalkanoates: A Versatile Class of Biopolymers, and Their Biomedical Potential, 1st Brazilian Workshop On Green Chemistry,18-21 November 2007, Fortaleza, Brazil
  • D. Yucel, H. Kenar, P. Sethu, N. Parashurama, V. Hasirci, D. Kaplan, M. Toner, Expression of Neural and Cardiomyogenic Lineage Markers by Mesenchymal Stem Cells from Human Bone Marrow and Wharton's Jelly (Poster Presentation), 2nd Annual Methods in Bioengineering Conference, 12-13 July, 2007, Boston, MA (USA)
  • D. Yucel, G. Kose, V. Hasirci, Influence of Physical and Chemical Cues onNeural Stem Cell Behavior on Tissue Engineering Scaffolds, 13th Biomedical Science & Technology Symposium, 26-28 August, 2007, İstanbul, TURKEY
  • H. Kenar, D. Yucel, D. Kaplan, M. Toner, G. Kose, V. Hasirci, Neural and Cardiomyogenic Lineage Markers Expressed by Wharton's Jelly Mesenchymal Stem Cells (Oral Presentation), 13th Biomedical Science & Technology Symposium, 26-28 August, 2007, İstanbul, TURKEY
  • D. Yucel, H. Kenar, E. Bella, G. Kose, C. Migliaresi, V. Hasirci, Effect of Polyester Type and Fiber Size on the Attachment ofWharton's Jelly Mesenchymal Stem Cells (Poster Presentation), 4th European Symposium on Biopolymers (ESBP2007), 2-4 October, 2007, Kusadasi, Izmir, TURKEY
  • H.Kenar,D.Yucel, E. Bella, G. Kose, C. Migliaresi , V. Hasirci. Effect of Polyester Type and Fiber Size on the Attachment ofWharton's Jelly Mesenchymal Stem Cells, (Oral Presentation), Annual Review Meeting of EXPERTISSUES, October 2007, Kusadasi, Izmir, TURKEY
  • V.Hasirci; H.Kenar; D.Yucel; P. Zorlutuna; P. Yilgor, B. Basmanav; B. Bayyurt; A. Ndreu; G. Torun Kose; N. Hasirci , Polymeric Nanobiomaterials in Drug Delivery and Tissue Engineering (Oral Presentation),International Workshop on Nanobiotechnology and Genome Technologies, October31- November 3, 2007, Antalya, Turkey
  • G. T. Köse, H.Kenar, D. Yücel, V. Hasırcı. Bone and neural tissue engineering, NationalArtificial Organs and Support Systems Congress (Oral Presentation), 15-17 June, İstanbul, Turkey
  • H. Kenar, D. Yücel, G. Torun Köse, V. Hasirci, Polycondensation polymers of natural and synthetic origin as biomedical materials, Koç Üniv. Istanbul 2006 (Invited talk)
  • D. Yücel, G. Torun Köse, V. Hasirci, Effect of Topography and Chemical Cues on Behaviour of Neural Stem Cells Regenerate World Congress on Tissue Engineering andRegenerative Medicine, Pittsburgh, PA (USA), 24-27 April, 2006