| Title: | Asst. Prof. ( Istanbul Technical University, Dept of Molecular Biology & Genetics) |
| Education: | Ph.D. Biotechnology, METU, 2001 M.Sc. Biotechnology, METU, 1997 B.Sc. Chemical Engineerring, METU, 1994 |
| Contact: | Phone : +90-212-285 7246 Fax : +90-212-285 6386 e-mail: kokf@itu.edu.tr Website: www.kokbiolab.com |
Research Field:
Immobilization of Biomolecules: Attachment of biomolecules, i.e. oligonucleotides, antibodies, receptors and enzymes, on different surfaces for design of biosensors, biocatalysts for reactors, biomimetic surfaces for cell adhesion. Development of new immobilization matrices, optimization of immobilization method and characterization of immobilized biomolecule activity are in the scope of this topic
Controlled Delivery of Bioactive Agents :Controlled delivery of bioactive agents is mainly used to have a better control on the concentration of the agent in the medium. Drugs, pesticides and fertilizers are incorporated generally in polymeric systems whose properties play an important role in the release behavior. The contact of the bioactive agent is limited with the environment and this prolongs, for example, halflife of a drug in the body or pesticide in the soil. In addition, this method minimizes the negative effect of drugs (e.g. cancer drugs) to untargeted tissues. Construction of controlled release systems from polymeric materials, determination of release rates depending on the polymer properties, preparation conditions and environmental factors are the scope of this research area.
Artificial Lipid Membranes on Solid Surfaces: Characterization and Usage in Membrane Protein Research Artificial lipid membranes on solid surfaces, on the contrary of other membrane models like liposomes, allow researchers to use surface sensitive techniques like Atomic Force Spectroscopy, Surface Plasmon Resonance Spectroscopy, Quartz Crystal Microbalance, etc. As well as being a good model for the membrane research, they provide the environment for functional incorporation of membrane proteins and their characterization. The effect of different compounds, inhibitors, drugs, on protein activity can also be studied by this method.
Selected Publications:
- E. Tastan, S. Onder and F.N.Kök, Immobilization of Laccase on Polymer Grafted Polytetrafluoraelthylene Membranes for Biosensor Construction, Talanta, accepted http://dx.doi.org/10.1016/j.talanta.2011.01.031
- S. Onder, K. Kazmanli and F.N.Kök, Alteration of PTFE Surface to Increase Its Blood Compatibility, Journal of Biomaterials Science, Polymer Edition, accepted http://www.ncbi.nlm.nih.gov/pubmed/20594420
- A.B. Ertan, P. Yılgor, B. Bayyurt, A.C. Çalıkoğlu, F.N. Kök, G.T. Kose, V. Hasirci, Effect of sustained growth factor release on cartilage tissue engineering, submitted
- E.-K. Sinner, U.Reuning, F.N.Kök, B.Saccà, L.Moroder, W.Knoll and D. Oesterhelt, Incorporation of integrins into artificial planar lipid membranes characterization by plasmon-enhanced fluorescence spectroscopy, Analytical Biochemistry, 333(2): 216-224, 2004
- F.N.Kök and V.Hasirci, Determination of binary pesticide mixtures by an acetylcholinesterase-choline oxidase biosensor, Biosensors and Bioelectronics, 19(7): 661-665, 2004
- F.N.Kök, F.Bozoglu and V.Hasirci, Construction of an acetylcholinesterase-choline oxidase biosensor for aldicarb determination, Biosensors and Bioelectronics, 17(6-7): 531-539, 2002
- F.N.Kök, F.Bozoglu and V.Hasirci, Immobilization of acetylcholinesterase and choline oxidase in on pHEMA membrane for biosensor construction, Journal of Biomaterials Science Polymer Edition, 12(11): 1161-1176, 2001
- F.Türesin, Z.Gümüsyazici, F.N.Kök, I.Gürsel, G.Alaeddinoglu, V.Hasirci, Biosynthesis of polyhydroxybutyrate and its copolymers and their use in controlled drug release, Turkish Journal of Medical Sciences, 30(6): 535-541, 2000
- F.N.Kök, R.M.Wilkins, R.B.Cain, M.Y.Arica, G.Alaeddinoglu and V.Hasirci, Controlled release of aldicarb from a lignin loaded ionotrophic hydrogel, Journal of Microencapsulation, 16(5): 613-623, 1999
- F.N.Kök, M.Y.Arica, O.Gencer, K.Abak and V.Hasirci, Controlled Release of aldicarb from carboxymethylcellulose microspheres in vitro and field applications, Pesticide Science, 55:1194-1202, 1999
- F.N.Kök, M.Y.Arica, C.Halicigil, G.Alaeddinoglu and V.Hasirci, Biodegradation of aldicarb in a packed bed reactor by immobilized Methylosinus, Enzyme and Microbial Technology, 24:291-296, 1999
- F.N.Kök, M.Y.Arica and V.Hasirci, Construction of a bioreactor for the degradation of pesticide aldicarb, Global Environmental Technology, 603-610, 1997
- F.N.Kök, M.Y.Arica, O.Gencer, K.Abak, and V.Hasirci, Controlled release of aldicarb from carboxymethylcellulose microcapsules, Turkish Journal of Chemistry, 21(2): 100-104, 1997
- F.N.Kök and V.Hasirci, Polyhydroxybutyrate and its copolymers: applications in the medical field, in: Tissue Engineering and Novel Delivery Systems, eds. M.J. Yaszemski, D.J.Trantolo, K-U. Lewandrowski, V. Hasirci, D.E. Altobelli and D.L. Wise, Marcel Dekker, Inc., pp 543-561, USA, 2004
- F.N.Kök, F.Bozoglu and V.Hasirci, Construction of an acetylcholinesterase-choline oxidase biosensor for aldicarb determination, in Novel Processes and Control Technologies in the Food Industry, eds. F. Bozoglu, T. Deak, and B. Ray, IOS Press, NATO Science Series, pp 49-62, Nederlands, 2001
- F.N.Kök, M.Y.Arica and V.Hasirci, In situ bioremediation of xenobiotics: immobilized biocatalysts in bioreactors, in: Bioremediation of Contaminated Soils, eds. D.L. Wise, D.J.Trantolo, E.J.Cichon, H.I.Inyang and U.Stottmeister, pp 133-150, Marcel Dekker, Inc., USA, 2000
Conferences
- F.N.Kök, B.Wiltschi, U.Reuning, W.Knoll and E.K. Sinner, Incorporation of Neuronal Acetylcholine Receptor Alpha7 into an Artificial Lipid Membrane, The 5th International Conference on Biological Physics, ICBP 2004, Gothenburg, Sweden, 2004
- D.Yucel, F.N.Kök and V.Hasirci, Construction of a teflon based HEMA-grafted enzyme biosensor, 18th European Conference on Biomaterials, ESB2003, Stuttgart, Germany, 2003
- F.N.Kök and V.Hasirci, Construction of an acetylcholinesterase-choline oxidase biosensor for determination of anti-cholinesterase compounds, 17th European Conference on Biomaterials, ESB2002, Barcelona, Spain, 2002
- F.N.Kök, F.Bozoglu and V.Hasirci, Bi-enzyme biosensor with acetylcholinesterase and choline oxidase, 8th Biomedical Science and Technology Symposium, Ankara, Turkey, 2001
- F.N.Kök, F.Bozoglu and V.Hasirci, Construction of an acetylcholinesterase-choline oxidase biosensor for aldicarb determination, The Sixth World Congress on Biosensors, San Diego, USA, 20006. S. Sehirli, F.N.Kök and V.Hasirci, Antibiotic release from PHBV22 implants, International Postgraduate Research Symposium on Pharmaceutics (IPORSIP 2000), co-sponsored by AAPS, Istanbul, Turkey, 2000
- F.N.Kök, M.Y.Arica, I.Gürsel, V.Hasirci and G.Alaeddinoglu, Biosynthesis of polyhydroxybutyrate and its copolymers by Alcaligenes latus, Biomedical Principles and Mechanisms of Biosynthesis and Biodegradation of Polymers, Muenster, Germany, 1998
- F.N.Kök, F.Bozoglu and V.Hasirci, Construction of a biosensor for the detection of the pesticides inhibiting acetylcholinesterase, 6th Biomedical Science and Technology Symposium, Izmir, Turkey, 1998
- F.Türesin, Z.Gümüsyazici, F.N.Kök, I.Gürsel, G.Alaeddinoglu, V.Hasirci, Biosynthesis of polyhydroybutyrate and its copolymers and their use in controlled drug release, 5th National Biomedical Science and Technology Symposium, Ankara, Turkey, 1998
- F.N.Kök, M.Y.Arica and V.Hasirci, Model drug release from hydrogel microspheres, 2nd National Biomedical Science and Technology Symposium, Ankara, Turkey, 1995
Awards
- Alexander von Humboldt Research Fellowship - 08/2003-10/2004
- First Poster Award - 8th Biomedical Science and Technology Symposium, Ankara, Turkey, 2001