BİOMATBIOMATERIALS AND TISSUE ENGINEERING RESEARCH LABORATORY


Assoc. Prof. Dr. Halime Kenar

 

 

Title:  Assoc. Prof. Dr. 
Education:  Ph.D. Biotechnology, METU, 2008
M.Sc. Biotechnology, METU, 2003 
B.Sc. Biological Sciences, METU, 2001
Contact:  Telephone:   
e-mail: halime.kenar@kocaeli.edu.tr

 

 

 

MSc Study:


In Vitro Bone Tissue Engineering On Patterned Biodegradable Polyester Blends

Cell alignment on PHBV-P(L/DL)LA microgrooved films had no effect on cell proliferation, while a significant improvement in osteoblast function  was obtained on fibrinogen  adsorbed micropatterned films 

 

Research Field:


3D Patterned Cardiac Tissue Construct Formation Using Biodegradable Materials

 

Heart failure, that can be caused by heart attack or congenital heart defects, is the number one cause of death in industrialized nations. One of the contributing factors in the progression of heart failure after heart attack is the loss of cardiomyocytes, cells of myocardium that contract to generate the blood pumping action of the heart. The injured myocardium is replaced by a fibrous scar, promoting the development of ventricular function loss, where currently allogenic heart transplantation is the only effective treatment. Unfortunately, heart transplantation is limited due to inadequate organ donations. Restoration of heart function by replacement of diseased myocardium with functional cardiac myocytes offers a potential cure. The elementary myocardial functional units are myocardial cells that are integrated into a multicellular assembly of myofibers aligned in parallel to each other. This study aims improvement of ventricular restoration procedures by providing a bioengineered graft that homes mesenchymal stem cells (MSCs) from umbilical cord matrix (Wharton's Jelly) aligned in parallel to each other on electrospun polymer microfibers in 3D architecture and biodegradable macroporous tubings used to supply growth media to the cells within the structure. The 3D construct obtained through tissue engineering approach and grown in a bioreactor demonstrated a uniform distribution of aligned viable cells for the duration tested (2 weeks). 




Academic Experience:

 

Nov 2005-Sep 2006 and Jun-Sep 2007: Scientific and Technical Research Council of Turkey International PhD Grantee and Visiting Researcher at Mass General Hospital/Shriners Hospital for Children at Harvard Medical School and TERC, Tufts U., USA 


Sept 2001- Sept 2008:  Research Assistant in Bioanalytical Chemistry and Genetics Laboratories, Middle East Technical University, Department of Biological Sciences

 

Selected Publications:

 

Journal Papers:


  • Yilgor P, Yucel D, Kenar H, Hasirci V. Polyhydroxyalkanoates: A Versatile Class Of Biopolymers, and Their Biomedical Potential, in press,  2008.
  • Kenar H, Kocabas A, Aydinli A, Hasirci V. Chemical and topographical modification of PHBV surface to promote osteoblast alignment and confinement. J Biomed Mater Res A. 2008; 85(4):1001-10.
  • Hasirci V, Kenar H. Novel surface patterning approaches for tissue engineering and their effect on cell behavior. Nanomedicine 2006; 1(1): 83-100.
  • Kenar H, Torun Kose G, and Hasirci V. Tissue Engineering Of Bone On Micropatterned Biodegradable Polyester Films. Biomaterials  2006; 27: 885-95.
  • Torun Kose G, Kenar H, Hasırcı N, Hasırcı V. Macroporous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) matrices for bone tissue engineering,Biomaterials 2003; 24(11): 1949-58.

 

Book Chapter:

  • Goktas H, Oke G, Kenar H, and Hasirci V. Plasma Based Electron Beam Generator and Its Nanobiomaterial Related Applications. Book chapter in Plasma Polymers and Related Materials (COST Action 527), Hacettepe University Press, Ed. in chief. Mehmet Mutlu, Eds. George Dinescu, Renate Förch, Jose Miguel Martin-Martinez, and Jiri Vyskocil, 2005.

 

Conference Abstracts:

  • Hasırcı V, Kenar H, Yucel D, Zorlutuna P, Yilgor P, Kose G, Hasırcı N. Collagen and polyester-based scaffolds for tissue engineering, 237th ACS National Meeting, "Genetically Designed Molecular Materials and Systems" Symposium, 22-26 March, Salt Lake City, UT, USA, 2009.
  • Kenar H, Yucel D, Kose GT, Hasirci V. Mesenchymal Stem Cell Behaviour on  Topographical Cues (Poster Presentation). TERMIS-EU 2008 Annual Meeting, 22-26 June, Porto, PORTUGAL, 2008.
  • Yucel D, Kenar H, Bella E, Kose G, Migliaresi C, Motta A, Hasirci V. Fabrication of Nano Size Polyester Fibers by Electrospinning (Poster Presentation). Trento Innovation Conferences in Materials Engineering (TICME), 1stMeeting: Advances in Polymers, Composites and Biomaterials, 16-19 December, Trento, ITALY, 2007. 
  • Yilgor P, Yucel D, Kenar H, Hasirci V. Polyhydroxyalkanoates: A Versatile Class of Biopolymers, and Their Biomedical Potential (Oral Presentation). 1st Brazilian Workshop On Green  Chemistry,18-21 November, Fortaleza, BRAZIL, 2007.
  • Yucel D, Kenar H, Sethu P, Parashurama N, Hasirci V, Kaplan D, Toner M. Expression of Neural and Cardiomyogenic Lineage Markers by Mesenchymal Stem Cells from Human Bone Marrow and Wharton's Jelly (Poster Presentation). 2nd Annual Methods in Bioengineering Conference, 12-13 July, Boston, MA, USA, 2007.
  • Yucel D, Kenar H, Bella E, Kose G, Migliaresi C, Hasirci V. Effect of Polyester Type and Fiber Size on the Attachment of  Wharton's Jelly Mesenchymal Stem Cells (Poster Presentation). 4th European Symposium on Biopolymers (ESBP2007), 2-4 October, Kusadasi, Izmir, TURKEY, 2007.
  • Hasirci V, Kenar H, Yucel D, Zorlutuna P, Yilgor P, Basmanav B, Bayyurt B, Ndreu A, Torun Kose G, Hasirci N. Polymeric Nanobiomaterials in Drug Delivery and Tissue Engineering (Oral Presentation). International Workshop on Nanobiotechnology and Genome Technologies, October 31- November 3, Antalya, TURKEY, 2007.
  • Kose GT, Kenar H, Yucel D, Hasirci V. Bone and neural tissue engineering (Oral Presentation). National  Artificial Organs and Support Systems Congress, 15-17 June, İstanbul, TURKEY, 2007.
  • Kenar H, Yücel D, Torun Köse G, Hasirci V. Polycondensation polymers of natural  and synthetic origin as biomedical materials (Invited talk), Koc Univ.,  İstanbul, TURKEY, 2006.
  • Kenar H, Aydinli A, Hasirci V. Micropatterned Polymeric Films Designed for Neo-Organ Preparation (Poster Presentation), Regenerate World Congress on Tissue Engineering and Regenerative Medicine, 24-27 April, Pittsburgh, PA, USA, 2006.